Vision Science 206B. Anatomy and Physiology of the Eye and Visual System
Structure and function of the tissues of the eye, ocular appendages and the central visual pathways; basic concepts of physiological, neurological, embryological, and immunological processes as they relate to the eye and vision; appreciation of the pathophysiology of various disease processes; importance of anatomy and physiology in the medical approach to ocular disease processes (continuation of VS 106A).
Vision Science 206D. Neuroanatomy and Neurophysiology of the Eye and Visual System
Structure and function of the neurosensory retina, photoreceptors, RPE including blood supply. Current concepts of etiology and management of major retinal conditions. Overview of diagnostic techniques in retinal imaging, electrophysiologic testing and new genetic approaches. Structure and function of the early visual pathway including retinal ganglion cells, optic nerves, lateral geniculate nucleus and visual cortex. Pupillary responses. Specialization in the visual cortex.
Vision Science 260C. Introduction to Visual Neuroscience
Retinal Neurobiology and Neurophysiology
We are interested in how visual signals are encoded and transmitted by neurons in the healthy retina and how signaling is perturbed during the course of retinal degeneration. Ongoing projects in the lab are addressing the following questions:
- How do retinal neurons extract specific features such as motion and spatial detail from the visual environment?
- How do different types of neurotransmitter receptors and ion channels shape the response properties of retinal neurons?
- How do mutations in the molecular machinery of cone-photoreceptors lead to retinal degeneration?
- How does the structure and function of the inner retinal circuitry change in response to photoreceptor degeneration?
To address these questions, we use a variety of research techniques including patch-clamp electrophysiology, immunohistochemistry, high-resolution imaging (confocal, super-resolution, electron and two-photon microscopy) and protein biochemistry.
Link to Pubmed Listing
Link to Google Scholar Listing
Gayet-Primo J, Yaeger DB, Khanjian RA, Puthussery T (2018) Heteromeric KV2/KV8.2 channels mediate delayed rectifier potassium currents in primate photoreceptors. J Neurosci 38:2440–17. * Cover Illustration
Gayet-Primo J. Puthussery T. Alterations in Kainate Receptor and TRPM1 Localization in Bipolar Cells after Retinal Photoreceptor Degeneration. Front Cell Neurosci. 2015. 9:486. doi: 10.3389/fncel.2015.00486. eCollection 2015
Balakrishnan V, Puthussery T, Kim MH, Taylor WR, von Gersdorff H. Synaptic vesicle exocytosis at the dendritic lobules of an inhibitory interneuron in the mammalian retina. Neuron. 2015 87(3):563-575.
Puthussery T, Percival KA, Venkataramani S, Gayet-Primo J, Grünert U. Taylor, W.R. Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina. J Neurosci. 2014 34(22):7611-21. PMID: 24872565; PMCID: PMC4035522.
Puthussery T, Venkataramani S, Gayet-Primo J, Smith RG, Taylor WR. NaV1.1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. J Neurosci. 2013 33(41):16045-59. PMID: 24107939; PMCID: PMC3792449. *Feature Article, *Cover Illustration
Puthussery T, Gayet-Primo J, Pandey S, Duvoisin RM, Taylor WR. Differential loss and preservation of glutamate receptor function in bipolar cells in the rd10 mouse model of retinitis pigmentosa. Eur J Neurosci. 2009 (8):1533-42. PMID: 19385989; PMCID: PMC2818147.